Global cycle properties in graphs with large minimum clustering coefficient

The clustering coefficient of a vertex in a graph is the proportion of neighbours of the vertex that are adjacent. The minimum clustering coefficient of a graph is the smallest clustering coefficient taken over all vertices. A complete structural characterization of those locally connected graphs, with minimum clustering coefficient 1/2 and maximum degree at most 6, that are fully cycle extendable is given in terms of strongly induced subgraphs with given attachment sets. Moreover, it is shown that all locally connected graphs with minimum clustering coefficient 1/2 and maximum degree at most 6 are weakly pancyclic, thereby proving Ryjacek's conjecture for this class of locally connected graphs.Comment: 16 pages, two figure

Words with many palindrome pair factors

Motivated by a conjecture of Frid, Puzynina, and Zamboni, we investigate infinite words with the property that for infinitely many n, every length-n factor is a product of two palindromes. We show that every Sturmian word has this property, but this does not characterize the class of Sturmian words. We also show that the Thue-Morse word does not have this property. We investigate infinite words with the maximal number of distinct palindrome pair factors and characterize the binary words that are not palindrome pairs but have the property that every proper factor is a palindrome pair."The first author is supported by an NSERC USRA, the second by an NSERC Discovery Grant."http://www.combinatorics.org/ojs/index.php/eljc/article/view/v22i4p2

NGC 6302: The Tempestuous Life of a Butterfly

NGC 6302 (The ''Butterfly Nebula'') is an extremely energetic bipolar nebula whose central star is among the most massive, hottest, and presumably rapidly evolving of all central stars of planetary nebulae. Our proper-motion study of NGC 6302, based on excellent HST WFC3 images spanning 11 yr, has uncovered at least four different pairs of expanding internal lobes that were ejected at various times over the past two millennia at speeds ranging from 10 to 600 km s^-1. In addition, we find a pair of off-axis flows in constant motion at 760 +/- 100 km s^-1 within which bright [Fe II] feathers are conspicuous. Combining our results with those previously published, we find that the ensemble of flows has an ionized mass > 0.1 M_sun. The kinetic energy of the ensemble, 10^46 - 10^48 ergs, lies at the upper end of gravity-powered processes such as stellar mergers or mass accretion and is too large to be explained by stellar radiation pressure or convective ejections. The structure and dynamics of the Butterfly Nebula suggests that its central engine has had a remarkable history, and the highly unusual patterns of growth within its wings challenge our current understanding of late stellar mass ejection.Comment: 17 pages, 3 figures, 1 tabl

Commercialising the production of Cobia in Australia

This project is a collaboration between the Department of Agriculture and Fisheries (DAF) and the Cobia aquaculture sector, predominantly Pacific Reef Fisheries (PRF). It was undertaken to consolidate the aquaculture in Australia of Cobia, a species offering considerable potential as a diversification option for pond-based culture activities in Queensland. The project addressed key elements of the production cycle in order to move industry towards a more commercial footing and advance both the scientific knowledge and human capabilities of the sector. The project involved DAF staff at the Department’s Bribie Island Research Centre (BIRC) and staff from the PRF farm in Ayr, North Queensland. From 2014-2017, research examined: health issues affecting Cobia in culture both in the hatchery and on-farm; the emerging issue of intersex in Cobia and potential for this to further impact production; capability development of PRF staff in preparation of the establishment of a commercial Cobia hatchery; and, the involvement of some new entrants to Cobia aquaculture utilising tank-based production systems

Forward K+ production in subthreshold pA collisions at 1.0 GeV

K+ meson production in pA (A = C, Cu, Au) collisions has been studied using the ANKE spectrometer at an internal target position of the COSY-Juelich accelerator. The complete momentum spectrum of kaons emitted at forward angles, theta < 12 degrees, has been measured for a beam energy of T(p)=1.0 GeV, far below the free NN threshold of 1.58 GeV. The spectrum does not follow a thermal distribution at low kaon momenta and the larger momenta reflect a high degree of collectivity in the target nucleus.Comment: 4 pages, 3 figure

Nuclear translocation of glutathione S-transferase π is mediated by a non-classical localization signal.

Glutathione S-transferase π (GSTπ), a member of the GST family of multifunctional enzymes, is highly expressed in human placenta and involved in the protection of cellular components against electrophilic compounds or oxidative stress. We have recently found that GSTπ is expressed in the cytoplasm, mitochondria, and nucleus in some cancer cells, and that the nuclear expression of GSTπ appears to correlate with resistance to anti-cancer drugs. Although the mitochondrial targeting signal of GSTπ was previously identified in the amino-terminal region, the mechanism of nuclear translocation remains completely unknown. In this study, we find that the region of GSTπ195-208 is critical for nuclear translocation, which is mediated by a novel and non-classical nuclear localization signal. In addition, using an in vitro transport assay, we demonstrate that the nuclear translocation of GSTπ depends on the cytosolic extract and ATP. Although further experiments are needed to understand in depth the precise mechanism of nuclear translocation of GSTπ, our results may help to establish more efficient anti-cancer therapy, especially with respect to resistance to anti-cancer drugs

Replication, Gene Expression and Particle Production by a Consensus Merkel Cell Polyomavirus (MCPyV) Genome

Merkel Cell Polyomavirus (MCPyV) genomes are clonally integrated in tumor tissues of approximately 85% of all Merkel cell carcinoma (MCC) cases, a highly aggressive tumor of the skin which predominantly afflicts elderly and immunosuppressed patients. All integrated viral genomes recovered from MCC tissue or MCC cell lines harbor signature mutations in the early gene transcript encoding for the large T-Antigen (LT-Ag). These mutations selectively abrogate the ability of LT-Ag to support viral replication while still maintaining its Rb-binding activity, suggesting a continuous requirement for LT-Ag mediated cell cycle deregulation during MCC pathogenesis. To gain a better understanding of MCPyV biology, in vitro MCPyV replication systems are required. We have generated a synthetic MCPyV genomic clone (MCVSyn) based on the consensus sequence of MCC-derived sequences deposited in the NCBI database. Here, we demonstrate that transfection of recircularized MCVSyn DNA into some human cell lines recapitulates efficient replication of the viral genome, early and late gene expression together with virus particle formation. However, serial transmission of infectious virus was not observed. This in vitro culturing system allows the study of viral replication and will facilitate the molecular dissection of important aspects of the MCPyV lifecycle

Ebola and Marburg Hemorrhagic Fevers: Neglected Tropical Diseases?

Ebola hemorrhagic fever (EHF) and Marburg hemorrhagic fever (MHF) are rare viral diseases, endemic to central Africa. The overall burden of EHF and MHF is small in comparison to the more common protozoan, helminth, and bacterial diseases typically referred to as neglected tropical diseases (NTDs). However, EHF and MHF outbreaks typically occur in resource-limited settings, and many aspects of these outbreaks are a direct consequence of impoverished conditions. We will discuss aspects of EHF and MHF disease, in comparison to the “classic” NTDs, and examine potential ways forward in the prevention and control of EHF and MHF in sub-Saharan Africa, as well as examine the potential for application of novel vaccines or antiviral drugs for prevention or control of EHF and MHF among populations at highest risk for disease

Right Hepatectomy in Patients over 70 Years of Age: An Analysis of Liver Function and Outcome.

BACKGROUND: As a consequence of the increase in life expectancy, hepatobiliary surgeons have to deal with an emerging aged population. We aimed to analyze the liver function and outcome after right hepatectomy (RH) in patients over 70 years of age. METHODS: From January 2006 to December 2009, we prospectively collected data of 207 consecutive elective hepatectomies. In patients who had RH, cardiac risk was assessed by a dedicated preoperative workup. Liver failure (LF) was defined by the "fifty-fifty" criteria at postoperative day 5 (POD) and morbidity by the Clavien-Dindo classification. Liver function tests (LFTs) and short-term outcome were retrospectively analyzed in patients over (elderly group, EG) and younger (young group, YG) than 70 years of age. RESULTS: Eighty-seven consecutive RH were performed during the study period. Indication for surgery included 90 % malignancy in 47 % of patients requiring preoperative chemotherapy. ASA grade &gt; 2 (44 vs. 16 %, p = 0.027), ischemic heart disease (17 vs. 5 %, p = 0.076), and preoperative cardiac failure (26 vs. 2 %, p &lt; 0.001) were more frequent in the EG (n = 23) than in the YG (n = 64). Both groups were similar regarding rates of normal liver parenchyma, chemotherapy and intraoperative parameters. The overall morbidity rates were comparable, but the serious complication (grades III-V) rate was relatively higher in the EG (39 vs. 25 %, p = 0.199), particularly in patients with diabetes mellitus (100 vs. 29 %, p = 0.04) and those who had additional nonhepatic surgery (67 vs. 35 %, p = 0.110) and transfusions (44 vs. 30 %, p = 0.523). The 90-day mortality rate was similar (9 % in the EG vs. 3 % in the YG, p = 0.28) and was related to heart failure in the EG. LFTs showed a similar trend from POD 1 to 8, and patients ≥70 years of age had no liver failure. CONCLUSIONS: Age ≥70 years alone is not a contraindication to RH. However, major morbidity is particularly higher in the elderly with diabetes. This high-risk group should be closely monitored in the postoperative course. Liver function is not altered in the elderly patient after RH